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KMID : 0616120100410040297
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Korean Journal of Pharmacognosy
2010 Volume.41 No. 4 p.297 ~ p.302
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The Studies on Identification of Lophatherum gracile(淡竹葉) Using AFLP fingerprinting Methods
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Shim Young-Hoon
Park Ju-Young
Cho Chang-Hee
Kim Ji-Yeon
Lee Jong-Hwa
Hyen Seong-Ye
Kim Sun-Ho
Kim Dong-Sup
Jang Seung-Yeup
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Abstract
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Lophatheri Herba is the aerial part of Lophatherum gracile Bronghiart(淡竹葉, Gramineae). 25~75 cm in length. Stem: cylindrical with nodes, empty inside, externally pale yellowish green. Leaf: dehiscent of lanceolate lamina, shrunken and rolled, 5~20 cm long, 10~35 mm wide; surface: pale green ~ yellowish green, parallel-formed with veins of square reticulate, more distinct of appearance on the lower surface. Banbusae Caulis In Taeniam is the stringy strip derived from the stem with the peeled-off epidermis of Phllostachys nigra Munro var. henosis Stapf, and Phllostachys bambusoides Siebold et Zuccarini (竹葉, Gramineae). Irregular in size and shape, thin plane ~ strip-shaped, sometimes powdery, sometimes 1~3 mm thick. Outer surface: pale green ~ yellowish green, sometimes grayish white L. gracile and P. nigra have different origins although they show similar morphologic features. We were able to distinguish between L. gracile and P. nigra which are almost indistinguishable through this study. AFLP(Amplifide Fragment Length Polymorphism) was more suitable for identifying differences between L. gracile and P. nigra in comparison with other genetic analysis using chemical analysis. Therefore. molecular biological methods are believed to be useful for discovering origins of herbal medicines.
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KEYWORD
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Lophatherum gracile, phyllostachys nigra, AFLP, PCR
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